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HPLC determination of desmosine and isodesmosine in tissues and its application to studies of alteration of elastin induced by atherosclerosis
Summary
Type:
HPLC
Application #:
107590
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
UV, 270nm
Conditions:
eluent: 0.1M phosphate buffer - acetonitrile (2.8:1, v/v) containing 20mM Na dodecyl sulphate, final pH 4.5
flow rate 0.8ml/min
Authors:
Yamaguchi Y
Source:
J Chromatogr 422 (1987) 53 - 59
Details
Legend: Fig. 3. Chromatograms of 1. IDE and 2. DES A) in a standard solution, B) in aorta and C) lung hydrolyzate samples, 50µl each injected, concentrations of IDE and DES A) both 5.0µg/ml, B) 4.9 and 7.0µ
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